Journal: International Journal of Molecular Sciences

Article Title: Exploring the Genetic and Clinical Landscape of Dedifferentiated Endometrioid Carcinoma

doi: 10.3390/ijms26094137

Figure Lengend Snippet: Immunohistochemical findings. ( A ) Hematoxylin and eosin staining showing WCs and UCs from case 9. ( B ) PAX8: expression in the WC and the loss of expression in the UC in case 7. ( C ) Cytokeratin: expression in the WC and the loss of expression in the UC in case 9. ( D ) Epithelial membrane antigen: expression in the WC and the loss of expression in the UC in case 9. ( E1 ) p53: wild-type expression in the WC and UC in case 9. ( E2 ) p53: overexpression in the WC and UC in case 7. ( F ) E-cadherin: expression in the WC and the loss of expression in the UC in case 7. ( G ) Fascin: the loss of expression in the WC and expression in the UC in case 9. ( H ) ARID1A: expression in the WC and the loss of expression in the UC in case 9. WC, well-differentiated component; UC, undifferentiated component.

Article Snippet: ARID1A , Santa Cruz Biotechnology (sc-32761) , 1:100.

Techniques: Immunohistochemical staining, Staining, Expressing, Membrane, Over Expression

Journal: PLoS Pathogens

Article Title: Identification of murine gammaherpesvirus 68 miRNA-mRNA hybrids reveals miRNA target conservation among gammaherpesviruses including host translation and protein modification machinery

doi: 10.1371/journal.ppat.1007843

Figure Lengend Snippet: The relative expression of endogenous mRNAs in non-infected versus infected B cells sorted from in vivo samples during chronic infection. Wild-type B6 mice were infected i.n. with 10 4 PFU of MHV68-H2bYFP. At 16 days, splenocytes were harvested and subjected to flow cytometric sorting to isolate both non-infected B cells (CD4-CD8-CD14-CD19+YFP-) and infected B cells (CD4-CD8-CD14-CD19+YFP+). Following sorting, the transcript level of MHV68 miRNA targets Arid1a and Ctsl were determined in each sample using qRT-PCR. Values represent the mean ± SEM of three independent experiments. Significance was determined by a two-tailed, unpaired t-test. ***p< 0.001.

Article Snippet: Approximately 10 μg of total protein was then separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE 10% gel), transferred to a nitrocellulose membrane and probed with antibodies directed to β-actin (Cell Signaling, 8H10D10), ARID1A (Novus Biologicals, NB100-55334), CTSL (R&D Systems, AF1515), EWSR1 (Abcam, EPR4647), IFITM3 antibody (R&D Systems, AF3377), or FOXJ3 antibody (R&D Systems, AF5786).

Techniques: Expressing, Infection, In Vivo, Quantitative RT-PCR, Two Tailed Test

Journal: Diagnostics (Basel, Switzerland)

Article Title: Relevance of ARID1A Mutations in Endometrial Carcinomas.

doi: 10.3390/diagnostics12030592

Figure Lengend Snippet: Figure 1. Immunohistochemical staining for ARID1A. (A) Preserved expression; (B) Loss of expres- sion. Magnification: 200×.

Article Snippet: ARID1A expression was evaluated using the rabbit anti-ARID1A polyclonal antibody (Atlas Antibodies AB, Sweden—1:90 dilution).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Diagnostics (Basel, Switzerland)

Article Title: Relevance of ARID1A Mutations in Endometrial Carcinomas.

doi: 10.3390/diagnostics12030592

Figure Lengend Snippet: Figure 2. ARID1A expression between wildtype and mutant patients by RNAseq. WT: ARID1A wildtype samples; MUT: ARID1A mutant samples.

Article Snippet: ARID1A expression was evaluated using the rabbit anti-ARID1A polyclonal antibody (Atlas Antibodies AB, Sweden—1:90 dilution).

Techniques: Expressing, Mutagenesis

Journal: Diagnostics (Basel, Switzerland)

Article Title: Relevance of ARID1A Mutations in Endometrial Carcinomas.

doi: 10.3390/diagnostics12030592

Figure Lengend Snippet: Figure 3. ARID1A expression between wildtype and mutant patients by qRT-PCR. WT: ARID1A wildtype samples; MUT: ARID1A mutant samples.

Article Snippet: ARID1A expression was evaluated using the rabbit anti-ARID1A polyclonal antibody (Atlas Antibodies AB, Sweden—1:90 dilution).

Techniques: Expressing, Mutagenesis, Quantitative RT-PCR

Journal: Diagnostics (Basel, Switzerland)

Article Title: Relevance of ARID1A Mutations in Endometrial Carcinomas.

doi: 10.3390/diagnostics12030592

Figure Lengend Snippet: Figure 4. Details of ARID1A Leu1100 (A), Asn1705 (B), Arg1833 (C), Arg1906 (D), and Leu2195 (E) and of the neighboring residues in the available structures. In each panel, the wildtype structure and the mutant calculated in silico have been reported on the left and on the right, respectively. ARID1A helices are in blue, while helices and strands from other SWI/SNF subunits are in light blue and in light yellow. The considered residue is in ball-and-stick representation while the residues in the vicinity are in sticks. The atoms are colored according to the atom type. The hydrogen bonds have been highlighted by using yellow dashed lines.

Article Snippet: ARID1A expression was evaluated using the rabbit anti-ARID1A polyclonal antibody (Atlas Antibodies AB, Sweden—1:90 dilution).

Techniques: Mutagenesis, In Silico, Residue

Journal: Frontiers in Oncology

Article Title: Downregulated ARID1A by miR-185 Is Associated With Poor Prognosis and Adverse Outcomes in Colon Adenocarcinoma

doi: 10.3389/fonc.2021.679334

Figure Lengend Snippet: Differential expression of ARID1A. (A) Differential ARID1A expression analysis across several cancer types (significant differences by the Mann-Whitney U test are marked with red*). Left, normal; right, tumor); (B) ARID1A expression include paired tumor and adjacent normal tissues from Gene chip data; (C) ARID1A expression include paired tumor and adjacent normal tissues from RNA-Seq data; (D) Significant different expression of ARID1A through metastases, normal, and tumors; (E–F) Experimental verification of ARID1A mRNA and protein levels in colon cancer cell line using qPCR and western blot, respectively.

Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline (1× TBS) containing 0.05% Tween-20, the membrane was incubated overnight at 4°C with rabbit antibodies against ARID1A (1:300, CUSABIO TECHNOLOGY), βactin (1:1000, Beijing Zhongshan Biotechnology Co., Ltd).

Techniques: Quantitative Proteomics, Expressing, MANN-WHITNEY, RNA Sequencing, Western Blot

Journal: Frontiers in Oncology

Article Title: Downregulated ARID1A by miR-185 Is Associated With Poor Prognosis and Adverse Outcomes in Colon Adenocarcinoma

doi: 10.3389/fonc.2021.679334

Figure Lengend Snippet: COAD patients with low ARID1A mRNA levels had a poorer prognosis and predictive value in the pathological stages of COAD. (A) Prognostic value of ARID1A in primary colon adenocarcinoma using Kaplan-Meier plotter from GEPIA2, OS, Overall survival, DFS, Disease free survival; (B) Prognostic value of ARID1A in metastases using Kaplan-Meier plotter from CanEvolve: M-OS, Metastases overall survival, M-DSS, disease-specific survival, and M-DFS, Metastases disease-free survival; (C) Pathologic M, pathologic N, and pathologic T, respectively; (D) Lymphatic invasion, microsatellite instability, and Vital status, respectively. * p < 0.05 and *** p < 0.001.

Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline (1× TBS) containing 0.05% Tween-20, the membrane was incubated overnight at 4°C with rabbit antibodies against ARID1A (1:300, CUSABIO TECHNOLOGY), βactin (1:1000, Beijing Zhongshan Biotechnology Co., Ltd).

Techniques:

Journal: Frontiers in Oncology

Article Title: Downregulated ARID1A by miR-185 Is Associated With Poor Prognosis and Adverse Outcomes in Colon Adenocarcinoma

doi: 10.3389/fonc.2021.679334

Figure Lengend Snippet: Mir-185 downregulates ARID1A in colon cancer. (A) ARID1A can be controlled by mir-185-5p; (B) Transcription factors that regulate ARID1A expression from Cistrome (Chip-Seq data); (C) Mir-185 is overexpressed in COAD; (D) ARID1A is negatively correlated with mir-185-5p in COAD; (E, F) Mir185 mimics and inhibitors results detected by qPCR; (G) Mir185 mimics and inhibitors results detected by western blot.

Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline (1× TBS) containing 0.05% Tween-20, the membrane was incubated overnight at 4°C with rabbit antibodies against ARID1A (1:300, CUSABIO TECHNOLOGY), βactin (1:1000, Beijing Zhongshan Biotechnology Co., Ltd).

Techniques: Expressing, ChIP-sequencing, Western Blot

Journal: Frontiers in Oncology

Article Title: Downregulated ARID1A by miR-185 Is Associated With Poor Prognosis and Adverse Outcomes in Colon Adenocarcinoma

doi: 10.3389/fonc.2021.679334

Figure Lengend Snippet: The correlation between ARID1A and immune cell infiltration. (A) The significant positive correlation between ARID1A and subtypes of immune cells (TIMER); (B) Kaplan-Meier curves for the immune infiltrates and ARID1A Expression. The correlation between ARID1A expression and the abundance of CD4+ T cells, B cells, and natural killer cells, respectively (TIMER).

Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline (1× TBS) containing 0.05% Tween-20, the membrane was incubated overnight at 4°C with rabbit antibodies against ARID1A (1:300, CUSABIO TECHNOLOGY), βactin (1:1000, Beijing Zhongshan Biotechnology Co., Ltd).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Downregulated ARID1A by miR-185 Is Associated With Poor Prognosis and Adverse Outcomes in Colon Adenocarcinoma

doi: 10.3389/fonc.2021.679334

Figure Lengend Snippet: Correlation analysis between ARID1A and gene biomarkers of immune cells in COAD (TIMER).

Article Snippet: After blocking with 5% non-fat milk in Tris-buffered saline (1× TBS) containing 0.05% Tween-20, the membrane was incubated overnight at 4°C with rabbit antibodies against ARID1A (1:300, CUSABIO TECHNOLOGY), βactin (1:1000, Beijing Zhongshan Biotechnology Co., Ltd).

Techniques: